BIOL 2070 Lecture Notes - Lecture 2: Neurospora Crassa, Bright-Field Microscopy, Lab Report
Document Summary
The experiment is designed to determine the effect of mycalolide b on the hyphae growth in the fungi neurospora crassa. Ho: 2nm mycalolide b has no effect on fungal tip growth and/or the position of the nuclei. Ha: 2nm of mycalolide b has an effect on fungal tip growth and/or the position of the nuclei. Through incubating the drug on a fungal infected agar cushions, and observing it under phase and fluoroscence microscopy. Use flat and non bubbled cushion slides: separate 4 slides into two groups; add 50ul growth medium to the control slides using a. Switch briefly to fluoroscence (fs14) briefly inbetween every 3 minute interval to note for any nuclei position changes. 5 trials and record all data and observations: switch back to fluoroscence microscopy (fs14) and align the ocular scale again between the nuclei and the tip of the hyphae to note down for any changes.
