BIOL 2040 Lecture Notes - Lecture 19: Tandem Repeat, Glutamine, Nitrogenous Base
Document Summary
Tandem repeats of variable length of short simple sequences cacacaca (between. Cag = gln ; microsatellite of cag = (cag)n. Ex: poly-q(gln)-disease: gene x -- 30(cag)30 end of gene x. Each mating produces individuals with unique microsatellite structures! Can be detected by pcr: denature: 96 anneal: 68 elongate: 72. Single nucleotide changed at given known location (known because we sequenced the genome) Present on average every 1000 base pairs; forensics can differentiate between people! These are population dependant; depends where ancestors are from. We can use pcr primers to determine snp"s by designing 4 primers per snp, each with different nucleotide (a, t, c, g) at the end. The one that sticks will show us which nucleotide is at that snp. Maximum of 4 snp"s at a given position; intuitive based on nucleotide possibiliies. **recall that 3gbp represents haploid # of genome. Triphosphate 5"(on single phosphate)-3"(on ribose sugar) how dna formed.
