BIOL 2030 Lecture Notes - Lecture 10: Ribose, Cell Nucleus, Nitrogenous Base

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Dna replication: dna replication is process where cell makes exact copy of its own dna (copy dna . Helicase: breaks the h-bonding b/t bases to sperate strands. Ssb: binds to single strands to prevent them from binding again. Dna primase: primer is required to bind at orgin. Primers are short rna sequences synthesized by primase. Dna polymerase 2: synthesis new strand by reading nucleotide on template strand. When polymerase 2 operates on ladding strand it creates okazaki fragments. Poly 2 reads the parent strand in 3 5; synthesizes new strand in 5" 3". Leading strand: strand that is synthesised towards the replication fork. Lagging strand: being synthesized away from replication force. Rna primers added by primase needs to be removed. Poly 1 removes rna primers and fills gaps with dna sequences. Okazaki fragments created on lagging strand are combined by dna ligase. Dna ligase joins strands of dna by catalyzing reformatting of phosphodiester bond.

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