BIOL 2021 Lecture Notes - Lecture 9: Copii, Golgi Apparatus, Lipid Bilayer Fusion
9 Feb 2017
School
Department
Course
Professor
Document Summary
Regulation of vesicle formation: lipid markers phosphoinositides, coat-recruitment gtpases, gtpase= gtp binding protein, gtp= active can bd made into gdp=inative using gtpase, molecular switches, gef = guanine nucleotide exchange factor = activates, gap= gtpase activating. Fig 13-14a: arf protein: copi coat and clathrin coats at golgi, sarl protein: copii at er. In its inactive form it is sar1 gdp and its activated by sar1 gef (which a membrane protein) Sar1 binds gtp: changes conformation so that it can bind to a membrane. Fig 13-14b copii vesicle formation: sec 23 and sec 24 proteins bind to sar 1, then we have cargo receptors that bind to adaptor proteins this creates the inner coat. Fig 13-2 budding use dynamin (specifically use clathrin vesicles) Question: rab proteins and snares identify vesicles and ensure the right vesicle fuse with the target. Question; how do the right snares and rabs get into the right membrane in the first place: a: no, a: we don"t know.
