BIOL 2020 Lecture Notes - Lecture 3: Buffer Solution, Sumo Protein, Methyl Group
29 Apr 2016
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For laboratory use, buffers are usually weak acids and their conjugate base. Buffer preparation: mix appropriate ratios of a weak acid and its conjugate base to achieve the desired ph (within the buffer region) Calculate how much acid/ base you need to get the right pka and ph: add enough strong base (eg. naoh) to a weak acid to bring the ph to the desired level (within the buffer region) 3. add enough strong acid (eg. hcl) to the conjugate base of a weak acid to bring the ph to the desired level (within the buffer region) Carbonic acid is the weak acid which dissociates to form conjugate base which is bicarbonate. Keeping bicarbonate levels high will help elevate the ph (clicker question) Higher concentration of protons will keep the equation moving in the direction of the. Many amino acids in proteins have side groups that can donate or accept protons, making them a signi cant source of buffer capacity.
