BIOL 1000 Lecture 10: Chapter 10 Genetic Recombination
21 Feb 2019
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Recombination occurs between similar regions in dna: homology allows different dna molecules to line up and recombine precisely. Once homologous regions of dna are paired, enzymes break a covalent bond in each of the four sugar-phosphate backbones. The free ends of each backbone are then exchanged and reattached to those of the other dna molecule. Final result is two recombined molecules: cutting and pasting four dna backbones= one recombination event. 10. 2 genetic recombination in bacteria: genetic recombination occurs in e. coli. E. coli and other bacteria can be grown in minimal medium. Detectable differences related to nutrition that occurred during changing combinations were used. Phototrophs: strains that were able to make the necessary amino acids. Auxotrophs: mutant strains that are unable to make amino acids, the required amino acid needed to be provided. X-rays), two different strains of e. coli were isolated which carried distinctive combinations of alleles for various metabolic genes.
