BIOL 1000 Lecture Notes - Multiple Cloning Site, Recombinant Dna, Sticky And Blunt Ends
25 Nov 2013
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Document Summary
Primer has opp ends of primes than the relication fork primes. ^ always elongated from the 3" to 5". Bacteria is much more easier to work with since the genome is smaller and similar to ours. Heterologous put dna into bacteria and can be used to express proteins that aren"t theres. Fermentation technology is well established go from small to large badges can work with milliletres and litres and do the same job. Cannot do glycolysis do not have golgi. Cannot remove introns unlike our dna (introns are removed) Polymerase chain reaction takes dna from breaking a cell open and copies is a lot of times. ^ template dna, primer, polymerase, and nucleotides are needed for this reaction. Recombinant dna dna from different organisms is combined into one organism. Denaturation heating temperature so that strands are separated. Bonds between as and ts will break since they have more bonds to be broken.
