HE440 Lecture Notes - Lecture 12: Tp63, Mdm2, P53
Document Summary
P53 and the dream complex: p53 controls extremely large number of genes involving both the up and downregulation of mrnas. Impairment of p53-dream leads to cancer phenotype: p53-dream targets include brca1, brca2. Immortality and telomere maintenance: hayflick limit the replicative potential of cells is limited by the shortening of telomeres, telomeres shorten by about 20bp each cell division and even as high as. Non-coding rnas: human genome: 1. 2% of dna is protein-coding genes, other 98/8% is noncoding, non-coding rnas include ribosomal, transfer, small nuclear, regulatory short and long non-coding rnas. Long non-coding rnas: 3" end of cdkn2a (cyclin dependent kinase inhibitors) encodes cdkn2bas an antisense non-coding rna in the ink4 locus (anril, anril is deregulated in many cancers - lung, breast, melanoma, nasopharyngeal, glioma. In normal cells: e2f induces anril to suppressed expression of the cell cycle inhibitors. Cdkn2a and cdknb: restart the cell cycle after dna repair. In cancer: hijacked and overexpression of anril prevents cell cycle halt during.
