Biology 1202B Lecture 23: Cycle-11
Document Summary
Rt-pcr: looking at cdna amount at the very end. Endpoint: run it on a gel, it"s qualitative. Qpcr: qpcr is not run on a gel, as it happens live, it plots it on a graph for you, starts at 0, number of cycles on the x axis. Only care to see if it can make mrna, don"t care about levels. qpcr. 3 lines per colour cause experiment is being done in triplicates: in order to increase reliability of results. Thursday, april 26, 2018: anytinhg below the threshold, you can"t qualitative to quantitate, the further left it is, the higher concentration it is. Three important factors allow for monitoring of pcr reaction in. Real-time pcr doing the same steps as pcr: denaturation, annealing, synthesizing(extnesion) Additional steps for qpcr: taq polymerase is not added, it"s a substitution for the regular taq. (amplitaq for the regular one). Has polymerization activity and 5" exonuclease activity.
