Biology 1002B Lecture Notes - Lecture 24: Experimental Evolution, Lte (Telecommunication), Growth Medium
Document Summary
Bio 1002b - lecture 24 experimental evolution i. Most studies of evolution are constrained by two facts: most organism evolve slowly, can"t go back in time to examine ancestral forms. Model systems for experimental evolution: viruses, bacteria, chlamydomonas, drosophila, yeast, what they all have in common short generation time. Compare sub population of evolved e. coli with original ancestral population. Start with single genotype of e. coli: split into two flasks, one flask is transformed with plasmid with neutral gene that codes for red pigment. This enables us to differentiate between two populations. Leave flask without plasmid to grow normally, under optimal conditions ancestral sample. Bombard coloured flask with x-rays to create mutations evolved sample. Place coloured population in low ph, see if they adapt in 2000 generations. Mix ancestral and evolved populations, subject them to low ph for few days: count initial cells. W (relative fitness) = growth rate in evolved strain/ growth rate in ancestral strain.
