Biology 1002B Lecture Notes - Lecture 20: Agrobacterium Tumefaciens, Ti Plasmid, Restriction Enzyme

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Lecture 18 basic mechanism of propagation of action potentials in neurons. Membrane at -70mv, na-k pump maintains this resting potential. A restriction enzyme cleaves sugar-phosphate backbones at its specific restriction site (in the bacterial plasmid) creating sticky ends. Fragments of genomic dna are produced using the same restriction enzyme which produces the same sticky ends as the restriction site. Dna fragments with the same sticky ends can pair therefore the genomic dna gets inserted into the bacterial plasmid. Nicks in sugar-phosphate backbones are sealed by dna ligase basic mechanism for creation of cdna. mrna is isolated from the cell. Polyt dna primer is added to base pair polya tail of mrna. Reverse transcriptase synthesize a dna copy in 5"-3" direction. A hybrid molecule consists of mrna base paired with a dna strand. Dna polymerase synthesize the second strand of dna ways in which cdna versions of genes would be different than genomic versions. cdna would not have a promoter region.

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