Biology 1002B Lecture Notes - Lecture 59: Organelle, Thymidine Triphosphate, Kleptoplasty
20 Apr 2016
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Interpretation of agarose gel data. Figure on the right is evidence that the transcript of psbo is actually produced in elysia. It provides evidence that the mrna corresponding to the psbo gene is being synthesized in. Pcr reaction cycle after 30 cycles 109 x amplification (billion fold) know direction of extension. The machine just cycles temperature. It adds 10 bases/second to the free three prime end of the free primer: pcr usually goes for 30 cycles. Extension (72 c: optimal temperature for dna polymerase to extend primers, role of primers in polymerase chain reaction. In any dna sequence there is a 1 in 4 change of finding an a, g, c or t. Therefore primers that are 16 bases long can be very specific. Primers are complementary and anneal to either end of the target sequence. Even if a sequence is very short, it is still very unique. One of the primers is bound to be correct: eg.
