Biology 1002B Lecture Notes - Lecture 11: Chlamydomonas, In Essence, Non-Competitive Inhibition

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The glucose is used in cellular respiration and the breakdown of glucose consumes oxygen and it generates co2. You can measure cellular respiration by measuring oxygen consumption. 1: how to measure carbon fixation in chlamydomonas. To measure co2 fixation in a culture, we measure the co2 fixation rate= molc/cell/min. Light intensity (x) vs. co2 (y) fixation rate. It goes progressively faster and faster because we know photosynthesis needs light. Co2 will fix it the plant is losing weight. The speed at which the enzymes in the calvin cycle can work (the speed of rubisco) ) enzymes differ remarkably in vmax. The measure of the interaction between the substrate and the enzyme is called km. Km is the substrate concentration that gets vmax. Km is a measure of affinity- the attractiveness between substrate and the enzyme complex. [lecture 9: integrated metabolism ii] If you are efficient at getting substrate and bringing it, you have a very low km.

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