Biology 1002B Lecture Notes - Lecture 14: Helix-Turn-Helix, Tata-Binding Protein, Leucine Zipper
Document Summary
Regulaion trna base pairs with o o o. Mutaion that leads to glucose having no efect on level of operon transcripion? o o o. So it no longer binds with camp, so it no longer binds to dna. During transcripion the polymerase uses complimentary base pairing with. Dna to know which base to add to the trna. Amino acids cannot base pair with anything laci-: consituive expression lacz-: never expressed. Lac operon expression is increased in presence of lactose. Be able to deduce where mutants likely are based on phenotype. Be able to predict phenotype of various mutants. Operon is under negaive control by dna binding protein. Repressor binds operator, makes loop in dna, prevening transcripion. Operon under posiive control by dna binding protein. Cap binding protein bends the helix, makes promoter more atracive o o o o o o o o o o expression.