Biology 1002B Lecture Notes - Lecture 8: Intermembrane Space, Glucose Transporter, Chlamydomonas
12 Mar 2016
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Document Summary
Cellular respiration use isolated mitochondria: insert picture of apparatus, grind up chlamy cells purify intact mitochondria incubate in bugger, insert picture. What is a heterotroph, what is an autotroph: autotrophs, organisms which can produce their own nutrients from surroundings i. e. photosynthesis, heterotrophs, rely on other organisms for food supply i. e. humans, cell respiration. What prevents chlamydomonas from being a heterotroph that grows on glucose: not multicellular, doesn"t have specialized cells to perform this function, glucose storage may inhibit cell functions, no access to glucose where it can get energy, no transporter on the plasma membrane for glucose. Can"t acquire from environment: means chlamy wouldn"t undergo glycolysis. Change in respiration rate (oxygen consumption ) in isolated mitochondria by addition of nadh, adp, uncoupler etc: environment, place in electrode, closed chamber, detects oxygen concentration. Concentration of protons in the intermembrane space (no atp synthesis) Gold: difference between cell respiration and photosynthesis.
