Biology 1002B Lecture Notes - Lecture 23: Chloroplast, Reverse Transcription Polymerase Chain Reaction, Symbiogenesis
31 Mar 2015
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Lecture 23b: horizontal gene transfer, moving genes between species. Lateral gene transfer: moving genes between organelles within an organism, kleptoplasty, kleptoplasts maintain photosynthetic competency, kleptoplasts functional for months after stealing them from other organisms where they would die within a day in other organisms. Location of psbo gene in photoautotrophic organisms: elysia: nuclear genes are being expressed and proteins imported into mitochondria. Gene that is normally found in the nucleus in algae is found in nucleus in. Protein is part of the oxygen evolving complex in thylakoid lumen. Interpretation of agarose gel data: agarose (runs dna gels, pcr product. Blast sequence to confirm if you have psbo: pcr primers find psbo in elysia genome. Gave way to a whole other algae (elysia) Vaucheria has 4 membranes around its chloroplast while other organisms have only two membranes: chloroplast membranes. They end up with two membranes meaning the psbo wont work.
