Biology 1002B Lecture Notes - Lecture 23: Sanger Sequencing, Synthetic Biology, Mycoplasma

• School: Western University
• Department: Biology
• Course: Biology 1002B
• Professor: Tom Haffie

Document Summary

Dna sequencing methods typically have these basic steps: purification, fragmentation, sequencing fragments, assembly of fragment sequences. Whole genomes can be sequenced by shotgun strategies: millions of copies broken into random pieces will give a bunch of overlapping fragments, computer takes fragments and then reassembles them into correct order, dna sequencing requires dna synthesis. Sager sequences uses four different reactions: old way to sequences used radioactive phosphorus and really long electrophoresis. Automated sanger sequencing uses a single reaction tube: way of sequencing dna, allows the whole genomes to be done at once (unlike before) can be done by a robot, makes sequencing far cheaper than ever before. Massive parallel systems process a billion dna fragments: innovation and automation worked together to drop the price, attaches the stuff to a solid surface, then makes multiple copies, sequencing them all at the same time, thousands of copies.