Biology 1002B Lecture Notes - Lecture 23: Minimal Genome, Synthetic Biology, Tata Box
16 Nov 2018
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Synthetic life: what is the fewest number of genes, minimal genome, all sequencing is synthesis, sequence dna from the complementary strand. Just because you think exons are more important, doesn"t mean they take up more space. Functional analysis: gene functions can be confirmed, altering expression (gene knockouts, transcriptomics, proteomics. Crispr: bacterial system that bacteria use as immunity, nuclease. Investigate problems that have no function at all: send it to cut chromosome, make guide rna that is complementary, cas9 system will make cuts, non-homologous end joining will try to repair. In/del mutation will occur: this is a way to make mutations, to degrade the chromosomes of infecting viruses, cas9 is a protein that is guided by single stranded rna, homology directed repair(hdr, genome editing. Instead of putting new genomes: still modified but not changing completely, can incorporate new dna into the genome, transcriptomics. Take mrna from tissue reverse transcribe to dna.
