Biology 1002B Lecture Notes - Lecture 8: Electrochemical Gradient, Atp Synthase, Electron Transport Chain

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Oxygen is consumed, popular way in measuring cellular respiration is the rate of consumption of oxygen. Adding nadh, oxygen consumption will go up, isolated mitochondria don"t have a source of substrate no pyruvate going into mitochondria, when you put nadh in, now has substrate. Quickly depletes levels of nadh within mitochondrial matrix, rate of electron transport (oxygen consumption) should go up. Adding adp+, rate of oxygen consumption goes up even more. *respiratory control: rate of electron transport will be dependent on availability of. *if there"s no adp around, proton gradient becomes very high; ph in intermembrane is very low, harder for complexes to pump protons into space with already a lot of protons, limits rate of electron transport. No substrate to drive phosphorylation of adp, high gradient, no adp in mitochondria, protons won"t flow back. *even though two independent processes (etc and atp synthase), they are coupled in a normal system, they influence each other.

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