Biology 1002B Lecture Notes - Western Blot, Nylon, Hexane
9 Sep 2013
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Two approaches: biochemistry looks at proteins to find biological function, genetics: understands the biological function by examining mutants, molecular biology: bought the two fields, biochemistry and genetics together. Translational control: trna recruitment and ribosomal level. Post transcriptional control (before translation): mrna decay- turnover. Abundance vs activity: abundance in protein doesn"t mean they are all active and functioning and expressing. Constitutive: gene is always on, contstant level of production, regardless of external changes (housekeeping genes, fundamental aspects of cell need constant supply of these) Induced or repressed: switched on under conditions/off usually on, but under conditions, they are turned off. Cell of tissue samples: isolate rna from each of them. Run rna on gel electrophoresis: separate rna based on size, smaller on the bottom, most abundant rna= ribosomal rna (97%), mostvisible on gel, each lane has 30,000 types of rna. Transfer to nylon membrane, thru capillary action, rna will transfer to membrane.
