Biology 1002B Lecture Notes - Lecture 20: Sanger Sequencing, Polyacrylamide Gel Electrophoresis, Mental Floss
16 Aug 2017
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Genomics consist of 3 main areas of study: genome sequence determination and annotation obtaining sequences of complete genomes and analysing them to locate putative protein-coding and noncoding. Rna genes and other functionally important sequences: determining the functions of genes and other parts of the genome. Comparative genomics studies of genome sequences for a number of organisms. Obtain sequence of bases in a genome using dna sequencing techniques . Analyse sequence using computer-based approaches to identify genes and other sequences of interest (gene regulatory sequences, origins of replication, repetitive sequences, and transposable elements) This sequence shows the beginning and end of a gene. If you wanted to pcr amplify only the coding region of this gene you would make the primer below: 5"atgaactc. This primer binds to the other dna strand at the same region but 3"tacttgag. So, something needs to bind on the other side of this dna strand.
