Biology 1002B Lecture Notes - Lecture 8: Inverted Repeat, Retrotransposon, Transposable Element
24 Oct 2016
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Transposable elements (te: move from place to play in genome, transposition- jumping genes . Transposition may be cut or copy and paste. Transposon must contact target site: they don"t actually jump . May lead to altered function of gene/ regulatory protein. Inverted repeat- end of te: transposase gene- catalyze transposition. Transposons have is flanking central sequence: may contain genes. First identified (but not understood) in corn: eukaryote. Cut and paste transposition: allow pigment gene to be expressed. Retrotransposons move via reverse transcriptase (copy and paste) Retrotransposons use reverse transcriptase (rt) to copy and paste. Retroviruses integrated in cell in proviral form: mutations in gag or env prevent formation of viral particle, functioning pol will allow for movement, retrotransposon is a partially/non functioning retrovirus. Rate of transposition events is low: sine 1/21 births (~4%, line 1/212 births (~0. 4%, sva1/916 births (~0. 1%) Of 970,000 lines: 100-170 retrotransposition competent. Snp or sequencing analysis of a single genome: compared to published literature.
