Biology 1001A Lecture Notes - Lecture 3: Post-Translational Modification, Opsin, Agarose Gel Electrophoresis

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Chin (covalent, hydrogen, ionic, and nonpolar) bonds form between r groups structure flexible, allowing different conformations: quaternary: more than one polypeptide chain in tertiary form come together to form complete, functional protein. Nuclear pores allow for transport in and out of nucleus. Protein abundance is controlled at the transcription and translation levels: transcription: dna needs to be copied to mrna, translation: taking mrna info and making a peptide chain. Measure protein abundance by measuring transcript abundance (dependent on life of mrna and rate of transcription) Measuring transcript abundance: northern blot analysis : Isolate rna from cell or tissue samples run on agarose gel, subject to electric field electrophoresis stain rna so you can see bands bands are rrna ribosomal bands of a prokaryote run to a different molecular mass. Transfer to nylon membrane because you can"t see specific rna on gel to measure specific rna, we have a single-stranded probe which has a radioactive label on it.

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