Biology 2581B Lecture Notes - Lecture 11: Arabidopsis Thaliana, Sacrum, Brachyury
19 Feb 2018
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Chapter 8 p 257, chapter 9 p 294-29, chapter 17 p 557-558. Reading what mutations tell us about gene function transposable genetic elements . Loss of function mutations reveal genes require from normal development. Early on, we relied mutants which either occurred spontaneously or were induced - random. Now, we like toe generate mutant with a speci c mutation in a speci c place, if possible. Rst sex linked recessive mutation ever discovered in the fruit y. Modern day use as a selective marker. Nude mice mutation disruption of the foxn1 gene. Impaired immune responses lack a thymus and cannot generate mature t lymphocytes e. g c. elegans. Rst suggestion to use this as a model organism. We need mutants other than morphological mutants e. g. mutants in biochemical pathways, subtle changes, timing, etc. Induced mutants increase frequency; larger variety. Ems (ethyl methanesulfonate) alkylating agent; popular mutagen. Ems causes g c to a t transitions (i. e. bp substitutions) subtle point mutations.
