Biology 2382B Lecture 1: Topic 1 - Intro To Cell Biology.docx
19 Jun 2015
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Overview of cell biology: cell structure, organelles and membrane trafficking, division and death, movement, signaling, communication. The cell: to study cells need to: Isolate and maintain cells in vitro: view them and know what to look for, separate cell organelles. Isolation and maintenance of cells: cells are isolated from tissue by breaking down the cell-cell and cell-matrix interactions (use mechanical fragmentation (scalpel), trypsin, edta) Spinning flasks used to prevent sticking of cultures: contact inhibition: too many cells prevent further dividing. Trypsin can be used to split: senescence: cells divide a limited number of times then a new sample must be taken. ~50 generations, hayflick limit: cell line are cells which are transformed and are able to grow indefinitely. Immortal : less likely to exhibit contact inhibition and have had an oncogenic mutation, the first human cell line called hela: henrietta lacks. Transformed cell: fibroblasts are the predominant cells in connective tissues, normal.
