Biology 2382B Lecture 2: lecture 2 - Imaging Cells .docx
26 Apr 2013
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Lecture 1 looking at how we get the cells, now we look at how we examine them, imagining them. 1600s people didn"t realize existence of cells. Anton van leeuwenhoek in early 1600s was the first person to build a microscope that was good enough to see cells. First to observe living protozoa and bacteria which he called animalcules . Went onto visualize human red blood cells and sperm. With great skill at grinding lenses naturally acute eyesight and lots of patient he was able to achieve a magnification of 200x. In contrast to anton"s microscop this uses multiple lenses as opposed to one. Light refraction the ability to bend light or to slow it down. Resolution: the ability to distinguish between two very closely position objects as separate entities. A conventional microscope can never resolve objects/cellular features that are less than ~0. 2 m apart. N: refractive index of medium between the specimen and the objective lens.
