Biology 2382B Lecture Notes - Lecture 7: Cyclin, G2 Phase, G1 Phase
27 Jun 2017
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G1 phase: generalized growth and metabolism of the cell, where most cells arrest when not dividing (g0, variable length (~11 hours in mammalian cells) G2 phase: preparation for chromosome segregation and cell division (~4 hours, some chromatin condensation. Higher number of cells in the g1 phase: If there is anything that indicates cells are not ready to divide the cell enters g0: inadequate nutrients, damaged dna, or just enter because they are post-mitotic cells. Mitosis: chromatin condense, so we can move chromatin easier, nuclear envelope breaks down, chromatids attach to mitotic spindle. If we have hela cells analyzed with flow cytometry and hoechst stain. We see double the amount of fluorescence (stain intensity) when dna is replicated (proportional) In the diagram we see the density of cells and dna in each phase of the cell cycle (g1, s, g2: more cells in g1 that have not replicated, least in s and some in g2.
