Biology 2382B Lecture Notes - Lecture 2: Differential Interference Contrast Microscopy, Phase-Contrast Microscopy, Fluorescence Microscope
11 Feb 2017
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First to observe living protazoa and bacteria which he called (cid:498)animalcules(cid:499) Went on to visualize human red blood cells and sperm. First person to ever see microorganisms: bacteria, etc. Bright-field microscopy: light source, condenser lens: focus light on specimen, objective lens: helps magnify light that has passed through the specimen. Passed through the instrument into the eye piece. Collect light after it has passed through specimen. No longer using a single lens to magnify using more than one (unlike anton: ocular and condenser lens = compound microscope (1+ lens) Helps magnify light that passes though a specimen. Light passes though the objective lens and magnifies again though the ocular lens. Total magnification is the product of the objective lens and the ocular lens: maximum magnification you can achieve is 1000x, ocular lens 10x magnification. If all the light passes though the biological organism unhindered, and you look into the eye piece, you.
