Biochemistry 3380G Lecture Notes - Lecture 9: Intermembrane Space, Electron Transport Chain, Stoichiometry
2 Feb 2015
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Proton gradient from electron transport: electron transport chain pumps protons out of the mitochondrial matrix gives a ph difference across the membrane, intermembrane space ph ~ 7, matrix ph ~ 7. 5. Chemiosmotic hypothesis: hypothesis that the protonmotive force drives synthesis of atp, explains why destruction of the proton gradient inhibits atp synthesis, creation of an artificial proton gradient stimulates atp synthesis. F1f0 atp synthase: a protein complex that allows protons to flow down their electrochemical gradient, the energy from this proton movement is used to make atp, atp is made in the matrix, coli f1f0 atp synthase. Red part is the part that spins, blue part remains stationary. F1f0 atp synthase: gamma subunit pushes on the outer subunits particularly the beta subunits which makes. Atp: c unit made of lipids and protons bind to it and rotate down through gamma, b subunit holds the alpha and beta stationary while beta and gamma rotates.
