Biochemistry 2280A Lecture Notes - Lecture 18: Phosphorylation, Plasmid, Agarose
6 Jan 2015
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Document Summary
Initiation: elongation, termination the amount of a mrna found in cell depnds on both its rate of synthesis and its rate of decay, which are both highly regulated, mrna degradation, protein modification, phosphorylation, acetylation, methylation, ubiquiylation, cleavage (insulin, protein degradation. Initiation transcription: rnap holoenzyme binds to the promoter (called closed complex when rna polymerase is bound to the promoter, rna polymerase unwinds the dna strands are the start site open complex, the first ntp is brought to the template no primer is required, using ntps; atp gtp ctp and utp as substrates, chain elongation proceeds in a 5" 3" directions following base pairing rules, after addidtion of 5 10 nucleotides, sigma falls off the holoenzyme, now the transcription bubble moves downstream (5" 3") with the template dna reannealing behind off, rna synthesis proceeds until a terminator is reached and rna polymerase falls, sigma rebinds rnap and the cycle is repeated.
