Biochemistry 2280A Lecture Notes - Lecture 23: Exon, Prokaryote, Consensus Sequence

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Derek Mc Lachlin

Document Summary

Youtube: ted talk richard resnick genomic revolution: the human genome: craig venter, francis collins, estimated to be a billion business. Traditional genome sequencing involves: 1) creating a genomic dna library, 2) many independent sequencing reactions, 3) aligning the independent sequences into a continuous sequence. Constructing a genomic library: total of 3 x 10^9 bp. G(cid:396)a(cid:373) (cid:374)egati(cid:448)e bacteria; genome ~ 2 million bp and circular: for 2 million bp, if you want the average insert size of 2000 bp, there must be at least. 1000 clones to cover the genome at least once. I(cid:374) this (cid:272)ase the(cid:455) did(cid:374)"t (cid:396)est(cid:396)i(cid:272)tio(cid:374) (cid:272)ut it; the(cid:455) so(cid:374)i(cid:272)ated it (cid:894)shea(cid:396) dna randomly in small pieces). So they had a coverage of around 20-fold of what was needed (minimally: now comes the sequencing. Isolated plasmids primers annealed just before the insert: that would read into the genomic dna, then they sequenced.