Biochemistry 2280A Lecture Notes - Lecture 2: Alpha And Beta Carbon, Acid Dissociation Constant, Anatomical Terms Of Motion

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Unit 1: fundamental concepts of biochemistry lesson 2: amino acids (sept. 8th 2017) Proteins are composed of amino acids based on genetic code: dna can encode 20 different amino acids to make up proteins. Ionization state influences a proteins structure and function. Ionization state is a function of: ph, affinity of the ionizable group for its proton (pka, environment (local) ph scale, ph = -log10[h+] = -log1010-7 = 7. Non-polar side chains: side groups cannot be ionized, cysteine can lose the proton and become and s- Polar, uncharged side chains: side groups cannot be ionized, tyrosine can lose the h and become o-. What is pka: only applies to weak acids and bases, ka = dissociation constant, personal scale of acidity, pka = -logka. Lower pka = stronger acid (low affinity for proton). At a given ph: calculate the extent of ionization of a group, from this, calculate the net charge on this group.

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