Biochemistry 2280A Lecture Notes - Lecture 23: Sequence Assembly, Organism, Contig
16 Jan 2018
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In the medical field, sequencing genes involved in disease can help people get personalized treatment, and help people would are at risk to take precautionary measures. Aligning the independent sequencing into a continuous sequence. Genomic dna library: a collection of cloned dna fragments that represent all of the dna in an organism"s genome. Dna are cleaved by restriction nuclease, partial digestion, fragments are inserted into plasmids using ligase and transformed in bacteria to get many independent clones. Genome was extracted and fragmented using sonicate (sound waves) to get fragments of various sizes. Dna was purified using gel electrophoresis, use sequences that are about 2000bp long. 4. clone library is prepared by inserting the dna it into plasmids and transformed into e. coli cells. Results in 20,000 clones each, each colony represents a fragment of genome. Sequence the ends of genomic clones using didoxy sequencing.
