Biochemistry 2280A Lecture Notes - Lecture 17: Lac Operon, Operon, Consensus Sequence
19 Dec 2017
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3 major topics: gene expression, recombinant dna technology, molecular basis of cancer. The central dogma: genetic info flows from dna to rna to protein (amino acids, dna is transcribes to rna then translated to protein. Clicker: at how many steps can gene expression be regulated in a eukaryotic cell: answer: >8. Transcription: transcription can be regulated in 3 different steps: initiaion, elongation, termination. Protein modification (only active when modified: phosphorylation, acetylation, cleavage (insulin) Protein degradation: the amount of a protein in a cell depends on both rate of synthesis and rate of decay, turn a protein over really quickly, it won"t get expressed. Transcription is the first step in the expression of any gene; it is usually the most highly regulated step to save energy. Prokaryotic transcription: gene and promoter structure, rna polymerase, mechanism of transcription, regulation of transcription, trp operon, lac operon. Promoter: the dna sequence required initiating transcription of a gene or operon.
