Biochemistry 2280A Lecture Notes - Lecture 25: Genomic Library, Contig, Francis Collins
25 Mar 2017
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Document Summary
Traditional genome sequencing involves: creating a genomic dna library (cloning, many independent sequencing reactions, aligning the independent sequences into a continuous sequence. Definition: a collection of cloned dna fragments that represent all of the dna in an organisms genome. Constructing a genomic library: have human genomic dna (lots of copies) Introduce the plamids into bacteria and get a genomic: 3 x 10^9 base pairs which are cleaved by restriction enzymes to get fragments that represent all of the dna in an organism. Dna fragments inserted into plasmids via ligase to make recombinant molecules library (vast amounts of clones. Clicker q: the h flue genome is 2 million base pairs. Take 2 million and divide by 2000 =1000. This is the abosulte minimum to cover the genom. To be sure you have the full coverage you want an excess of several fold. Genome circular like ecolie 92 million base pairs approx. )
