Biochemistry 2280A Lecture Notes - Lecture 25: Dna Ligase, Gene Knockout, Dna Profiling
25 Mar 2017
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Document Summary
Think that an aspartate within your favourite protein may lead to its proteolysis. To over come this you want to change the aspartate codon to one for alanine. Taper with a gene by customizing it. Creating a gene with altered sequence and in turn altered function for protein. Converting a codon for asp to a codon for ala. Three different types of genetic change possible with gmo: gene knockout. Adding antifreeze coding gene to a plant crop gene: both genes active. Analysis of bacteria or viruses in clinical or environmental samples. Diagnosis of genetic disease or in genetic counselling. Patient comes in, suspect they have hiv- test for it. Signature for virus is the viral genome, if an find the viral genome in patient means that their infected. From the cells can isolate for the rna (hiv is an rna virus) Make cdna via reverse transcription and then do pcr of the hiv cdna.
