Biochemistry 2280A Lecture Notes - Lecture 19: Thalassemia, Hbb, Hemoglobin

• School: Western University
• Department: Biochemistry
• Course: Biochemistry 2280A
• Professor: Boffa/ Mc Lauchlin/ Brandl

Document Summary

5" capping: 7-methylguanosine at the 5" end. Cap marks the 5" end of the mrna as being. Translation of the mrna intact: almost all auk mrnas end with a long (~base) Marks the (cid:885)" end of the mrna as being intact. Rna splicing: protein encoding sequences (exons) are interrupted by noncoding sequences (introns) )ntrons are (cid:498)spliced(cid:499) out of the primary transcript to give the mature mrna: exons = expressed. Splicing increases the coding capacity of the genome. Differential splicing: an rna can be spliced in diff ways that will create related but distinct proteins. Almost always includes exon 1 so you cant skip it: the splicing pattern is often tissue specific. Difference in the 2nd exon and difference in the (cid:885)" most exon. They have unique differences: mechanism of splicing. 3) branch point: there is an adenine which is crucial, the sequence is crucial for splicing. Introns are removed in two consecutive transesterification reactions.