Biochemistry 2280A Lecture Notes - Lecture 18: Protein C, Fatty Acid Metabolism, Atp Synthase

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We need to import phosphate and adp into the matrix and export atp out. These processes are driven by the proton motive force using energy of 1 proton. Phosphate import is driven by the proton movement across the membrane (doesn"t affect charge) Atp/adp anti-porting system operates on charge difference, not on proton movement: stoichiometry of atp synthesis. Assume: atp synthase uses 3 protons to make 1 atp (actually takes 3. 3 but round down) So: total cost of 1 atp = 4 protons across the membrane: nadh made in the matrix causes the pumping of 10 protons out of the matrix. So/ whenever you synthesize one nadh, it"s worth 2. 5 atp: oxidation of fadh2 or cytosolic nadh pumps 6 protons = worth 1. 5 atp, 2 nadh are made per glucose (glycolysis 2 atp directly) Nadh can"t cross the membrane so it gives up its electrons to the purple complex: know table 14-1 in the book.

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