Biochemistry 2280A Lecture Notes - Genomic Library, Intron, Tandem Repeat
7 Dec 2012
School
Department
Course
Professor
Document Summary
Method for identifying individuals and is done through comparison of multiple hypervariable regions in dna. There are dinucleotide repeats within our genome of (ca)n where n = 4 40: note that n varies in different individuals. Such repeats occur (with different flanking sequences) about 100,000 times in the human genome - are called vntrs (variable number of tandem repeats) To fingerprint an individual, you use primer pairs surrounding a number of these repeats. In many regions of our genome, there are repetitive sequences (cacacaca ) The repeat varies from ~4-40 times depending on the allele. Vntr the repeat sequences are called vntrs for variable number of tandem. Each vntr is amplified by pcr and analyzed by agrose gel electrophoresis. Pcr products with differing numbers of repeats will run at different positions on the gel. The profile for all the repeats analyzed represents the fingerprint for that individual.
