Biochemistry 2280A Lecture 6: Enzyme Catalysis Part I Sept 21
Document Summary
Types of chemical reactions carried out by enzymes: hydrolytic cleave using water, nucleases, proteases, phosphatases, e. g. thrombin, condensation connect molecules together, polymerases, synthases, e. g. Isomerases: oxidation-reduction gain or loss of electrons, oxidases, reductasaes, de- hydrogenases, e. g. Alcohol dehydrogenase: group transfer transfer chemical group, phosphorylation, sumoylation, Acetylation, glycosylation (n/o linked: myosin light chain kinase and phosphatase, lysine acetyl transferase, histone deacetylase. Part of the enzyme where the reaction takes place: small part of enzyme surface (5% or less, often a cleft or crevice between domain. Substrate binds in active site: binding is made of weak bonds, binding is reversible. Reaction coordinate: a map of the progress of the reaction. Enzymes make it easier to get to the transition state. Enzymes specifically aim to lower the activation energy rather than just adding energy through other methods such as heat. Enzymes do not change the but decreases the activation energy: