Anatomy and Cell Biology 3309 Lecture Notes - Lecture 20: Buffy Coat, Bone Marrow, Blood Film
5 Apr 2018
School
Department
Professor
Document Summary
Blood cells all have a limited lifespan, there has to be a process for replacement. Red bone marrow is where majority of blood formation occurs. There are various stages from immature precursor cells to the circulating cells where the cells can undergo division and morphological changes: recognize these on a smear of bone marrow. Megakaryocytes cells with large nuclei that give rise to platelets. Take blood that you have anti-coagulated, spinning it done at a low speed: buffy coat all the formed cells within the blood stream besides rbc, rbc on bottom. Took bone marrow out of animals + separated out the cells into individual cells in suspension & plated it with agarose. Cells would divide and give rise to colonies of some cell type. Determined that there is a pleuripotential stem cell that could produce everything and itself: knew the cell had to exist because it was a single cell in the agarose, but they did not what.
