MICR 302 Lecture Notes - Lecture 5: Reverse Transcriptase, Ribonucleotide Reductase, Northern Blot
7 Apr 2018
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Traditional molecular biology: analysis of individual genes, proteins, molecules by studying their speci c functions. Systems biology: the study of an organism as an integrated and interacting network of genes, proteins, molecules and biochemical reactions requires systematic data collection for all molecules in a cell. Emerged a lot in the late 90"s/early 2000"s when the internet and computers emerged, and the rst yeast genome was sequenced. Comprehensive study of the mrnas measured by northern blot. Add a mating pheromone and a labelled 32p nucleic acid probe to a lane with the mrna (hybridizes with the mrna and detects mrna from. One gene at a time, needs a hypothesis to be able to design the correct probe. Microarrays = look at the quantity of all the transcripts in the cell. Involves the isolation of mrna, puri cation (based on the presence of the poly a tail just add a poly t).
