PHY100H1 Lecture Notes - Lecture 5: Dimethylglyoxime, Paper Towel, Potassium Thiocyanate
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PHY100H1 Full Course Notes
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The first use of chromatography was described by the russian botanist, mikhail tsvet, in: he used a solvent to carry coloured material extracted from vegetables along a length of paper and demonstrated the separation of - and -carotene. Tsvet named the process chromatography, from the greek for colour writing (chromos and graph). Today the term applies to a number of methods for separating the components of a mixture on a support material. In paper chromatography, a sample spot of the mixture to be analyzed is applied on to an adsorbent paper (the stationary phase). The end of the paper is then dipped into a solvent (the mobile phase) which then rises up the paper by capillary action. As the solvent passes over the sample spot, the components of the mixture are attracted to the solvent and are carried with it up the paper.
