MGY377H1 Lecture Notes - Lecture 19: Biofilm, Membrane Technology, Polysaccharide
1 Feb 2018
School
Department
Course
Professor
Document Summary
The bacteria secrete out enzymes that will cut up massive molecules that cant get across the membrane. If they put the enzyme on the biofilm, the sugars cant diffuse away and they are concentrated so they can easily be used. Thus the biofilm becomes a digestive organ for the entire column. We stud(cid:455) (cid:271)iofil(cid:373)s (cid:449)ithout (cid:373)i(cid:373)i(cid:272)ki(cid:374)g the e(cid:374)(cid:448)i(cid:396)o(cid:374)(cid:373)e(cid:374)t that the (cid:271)a(cid:272)te(cid:396)ia a(cid:272)tuall(cid:455) li(cid:448)es i(cid:374), a(cid:374)d (cid:449)e a(cid:396)e(cid:374)"t do it polymicrobially, we are doing this in monocultures, with isolated bacterial species. Genetics told us that in this setup, theres 3 proteins and a polysaccharide called vps that go into making a (cid:271)iofil(cid:373). When the cell first lands, they produce rbma, then bap1 is produced, then rbmc and then after 270m, the(cid:455) (cid:373)ake a (cid:373)atu(cid:396)e (cid:373)i(cid:272)(cid:396)o(cid:272)olo(cid:374)(cid:455). If the(cid:455) do(cid:374)"t (cid:373)ake the pol(cid:455)sa(cid:272)(cid:272)ha(cid:396)ide, the(cid:396)es (cid:374)o a(cid:272)(cid:272)u(cid:373)ulatio(cid:374) of r(cid:271)(cid:373)a, bap1 a(cid:374)d r(cid:271)(cid:373)c. these proteins have carbohydrate binding domains on them. In the end, they saw that bap1 forms the floor of the biofilm.
