CSB332H1 Lecture Notes - Lecture 16: Patch Clamp, 5-Ht3 Receptor, Ketamine
22 Mar 2013
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Document Summary
Recap: rapid anti-depressant treatment including ketamine and dbs. This slide shows excitatory post syn currents on the post syn pyramidal neuron in response to administration of ketamine in comparison to control. Measuring the amount of current/ions that goes into the neuron in a patch clamp experiment. Able to quantify the total current going into the neuron in response to diff stimulations. First applied 5ht in bath solution to be able to open all 5ht activated channels/receptors. 1 is permeable to na current; open -> fast rapid influx of na. Also g-protein coupled receptors activated by serotonin; also induce increases in diff types of ionic currents (inward) Functional effect of ketamine; electrophysiological indication that ketamine causes an increase in synaptogenesis -> putative mechanism. Also did whole cell patch clamp recording. But they weren"t looking at synaptic transmission or transmission in response activation of receptors by ligands, serotonin etc.
