CSB349H1 Lecture Notes - Lecture 3: Euchromatin, Chromatin Remodeling, Repressor

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29 Mar 2017
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Genome sequencing: we determine the order of a, c, g, and t, dna sequencing technology has improved rapidly and the cost has also been reduced, the major technique being used today is sequencing by synthesis (illumine) They have special dna bases and when the bases join into a growing chain, they emit light and they make it so a, c, g, and t make different colour of light. They synthesize the dna and see what kinds of light is emitted. By recording the light, they can determine the sequence of letters. You can make a machine that can do this really quickly and in parallel. You can sequence millions of dna sequences at the same time. You ligate onto the sequences adaptors which are little bits of. Dna that will stick to a surface (silicon) that have primers. Adaptors will stick onto the primers and the dna fragments will attach to the surface.

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