CSB328H1 Lecture Notes - Lecture 23: Bone Morphogenetic Protein 2, Sox9, Limb Development
28 Aug 2019
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Document Summary
Does a turning-type mechanism cause the alternating pattern of digital and interdigital zones: sox9 is a transcription factor. Sox9 expression in growing limb buds: number of sox9 positive stripes increase with increase with increasing size of autopod, graph shows sox9 distribution in autopod (measured along dashed line in limb bud) Alternating pattern of sox9 positive and negative cell groups emerges after 20hrs. Reaction-diffusion models: what is the minimum factors they need, need at least two factors to generate a wave pattern, second factor can be expressed in phase or out of phase , transcriptomic profile: Looking for genes that will be only expressed in sox+ cells and not in sox- cells and vice verse. Found that only a few genes followed that pattern. Bmp and wnt signaling during digit formation: psmad only in sox9 area (digital zone) Alternating expression of sox9 and bmp2 in the developing autopod: bmp2 and sox9 are out of phase at all stages of autopod development.
