CSB328H1 Lecture Notes - Lecture 13: Myogenin, Myf5, Myf6
28 Aug 2019
School
Department
Course
Professor
Document Summary
Lecture 13: myogenic factors and muscle stem cells. Muscle development: muscles arise from somites and they are induced to develop muscle precursors through cell signalling, specification/determination of myoblasts (precursor cell) Blasts means that they are already actively dividing cells: proliferation, migration of myoblasts, differentiation into muscle fibers consists of multiple steps (terminal differentiation, muscle specific proteins myosin and actin. Functional analysis of mrfs: muscle regulatory factors: myod, myf5, mrf4, myogenin (potent transcription factors, gain of function assay cell culture (in vitro) Ectopic expression express a molecule when it is not normally expressed or at a time that is not expressed at (spatially and temporally different expression) Each myogenic factor by itself is sufficient to drive muscle development in vitro. Loss of function assay mouse mutant/knockout/reduced gene function using rnai (in vivo) Myf5 and myod control the determination of myoblast fate. Removal of myf5 or myod, nothing happens (redundant in gene function?)
