CSB328H1 Lecture Notes - Lecture 9: Embryonic Stem Cell, Inner Cell Mass, Different Fur
25 Oct 2016
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Embryo is transplanted into the foster mother. Result: brown mice: carried the genetic information from the nucleus donor. Different fur: used as a control. Genotypic tests were also done to see results. Fertilized egg can give rise to all cell types = totipotent cell. Differentiated somatic cell or tissue-specific stem cells = only one or few cell types. This tells us that (2 messages: differentiated cells carry the genetic material to create the whole organism. U(cid:374)til the(cid:374), it (cid:449)as(cid:374)"t (cid:272)lear (cid:449)hether all of our so(cid:373)ati(cid:272) (cid:272)ells (cid:373)ai(cid:374)tai(cid:374)ed the same genetic materials as the zygote. Cytoplasm is important for the totipotency of the cell. Inner cell mass: gives rise to the embryo, embryonic stem cells (es) = pluripotent stem cells, can culture these cells, can be kept in culture with embryonic stem cells. Wanted to see whether or not he can produce pluripotent stem cells from differentiated cells: the experiment before showed pluripotent stem cells derived from early embryonic stem cells.
