BCH311H1 Lecture 3: BCH311 – Lecture 3

• School: UTSG
• Department: Biochemical Engineering and Applied Chemistry
• Course: BCH311H1
• Professor: Stavroula Andreopoulos

Document Summary

Nucleotides: recall: nucleotide has phosphate nucleoside is base and sugar. Nomenclature: ribonucleotide, adding a single phosphate to a ribonucleoside, thymidine, one exception, with base as thymine, drop the deoxy as it never actually occurs in the hydroxylated form (2" hydroxyl is missing, never found in rna. Intermediate: tetrahydrofolate acting as an h donor common intermediates. Production of carbamoyl phosphate: start with bicarbonate, phosphorylate it with atp by transferring a phosphate onto it, have an amino group from glutamine and a phosphate is released and carbamic acid is formed. Carbamoyl phosphate synthetase: large multi-domain enzyme, has the ability to catalyze the 3 reactions, 3 large domains where the 3 active sites take place, yellow is the path where the intermediates move. Feedback regulation of pyrimidines: ctp acts as a regulatory molecule, atpase is an example of allosteric regulation, when binding can activate/inactivate an enzyme, biosynthetic - therefore need energy and as phosphate donors, atcase, regulated by our final product.